Immunocytochemistry and Immunofluorescence Staining Protocol
Immunocytochemistry and Immunofluorescence Staining Protocol
immunocytochemistry
5x Buffer PB (= 250 mM phosphate buffer, pH 7.2 after dilution)
To make 500ml,
Mix 140ml 0.25M NaH2P04
+ 360ml 0.25M NaH2P04
OR
1x PB, 500ml
35 mL of 0.2 M NaH2P04
90 mL of 0.2 M NaH2P04…