Immunoprecipitation Pull-Down Assay Protocol Using Protein A or Protein G Beads
Immunoprecipitation Pull-Down Assay Protocol Using Protein A or Protein G Beads
washing buffer
20mm Tris
0.2% Triton
elution buffer
made in the same buffer as washing buffer, but also containing 4% SDS (and also included a protease inhibitor if required)
1. centrifuge debris on the right ultracentrifuge at 100,000 x g …