Restriction Enzyme Digestion of Plasmid DNA

Restriction endonuclease Digestions – volume of use:

DNA 2uL (enough to check minipreps)
10x buffer 1UL (use 1.5uL to 15 digestions uL, and so on)
enzyme 1UL
water to 10ul

digest for at least 1 hour; if the quantitative digestion …